<i>Tnfα</i> mRNA is strongly induced in adipose tissue macrophages of <i>aP2-Gpr43TG</i> mice.

<p>(a) <i>Tnfα</i>, <i>F4/80</i>, and <i>Il6</i> mRNA expression in the epididymal adipose tissue SVF of control WT and <i>aP2-Gpr43TG</i> mice fed an NC measured using qRT-PCR (n = 3). (b) Representative flow cytometric plots (left) and bar graphs summarizing the data for CD11b⁺F4/80⁺ expression by live CD45⁺ cells (right) extracted from the epididymal adipose tissue of control WT and <i>aP2-Gpr43TG</i> mice (n = 3−4). R4: CD45⁺CD11b⁺F4/80⁺ cells, R5: CD45⁺CD11b^-F4/80^- cells. (c) <i>Tnfα</i> mRNA expression in epididymal adipose tissue MAs, macrophage subsets, and non-macrophage subsets of <i>aP2-Gpr43TG</i> mice fed an NC, measured using qRT-PCR (n = 4). (d) <i>GPR43</i> mRNA expression in epididymal adipose tissue MAs, macrophage subsets, and non-macrophage subsets of control WT or <i>aP2-Gpr43TG</i> mice fed an NC, measured using qRT-PCR (n = 3−4). <i>18S</i> mRNA expression was used as an internal control. Mice were analyzed at 35–40 weeks of age. All data are presented as mean ± S.E.M. *<i>p</i> < 0.05, **<i>p</i> < 0.01, ***<i>p</i> < 0.001, N.S.: not significant.