Obese white adipose tissue (WAT) gene expression and impact on macrophage migration.

<p>(<b>A</b>) WAT derived from wild-type (wt) and obese ob<i>/</i>ob mice was subjected to qRT-PCR. Increased gene expression of CD68, MCP-1, furin, MT1-MMP, and PCSK5 was found in ob<i>/</i>ob mice (*p<0.05; **p<0.01 vs. wt). (<b>B</b>) Supernatants from WAT cultures of ob<i>/</i>ob mice increased THP-1/ϕ migration (^##p<0.05 vs. wt), comparable to MCP-1 (*p<0.05 vs. control, co.; **p<0.01 vs. 50 ng/mL MCP-1). (<b>C</b>) Supernatant-facilitated migration was inhibited by dec-CMK (CMK, 50 µmol/L; 12h) or the MMP-inhibitor GM6001 (50 µmol/L; 12h) (both #p<0.05 vs. controls, co.). n=3.