Inflammatory gene expression profiles of SVF and adipocytes from WT and HO-1^+/−chimeras.

<p>Chimeric mice transplanted with WT or HO-1^+/− bone marrow were fed a regular chow (RC) diet or a HFD for 36 weeks, then the animals were sacrificed, the visceral adipose tissues collected, and the SVF and adipocytes isolated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038626#s4" target="_blank">Materials s and Methods</a>. The expressions of the indicated genes in the SVF (A) and adipocytes (B) were then quantified by real time RT-PCR. The expression level of 36B4 gene was used as an internal control for normalization. The number of animals in each group was four to seven. <i>P</i><0.05 vs the chow diet-fed group of the same genotype; ^†<i>P</i><0.05 vs the HFD-fed WT group. C, The protein levels of proinflammatory cytokines in extracts of adipose tissues were determined by ELISA as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038626#s4" target="_blank">Materials and Methods</a>. <i>P</i><0.05 vs the chow diet-fed group of the same genotype; ^†<i>P</i><0.05 vs the HFD-fed WT group.