Validation of RNA sequencing by RT-qPCR.

<p>Graphs showing fold changes between 4-cell/2-cell stages derived from ddCt values (black bars; qPCR) and identified by RNA sequencing (green bars; RNA-seq). <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061547#pone-0061547-g001" target="_blank">Figure 1A</a> shows the validation results for genes identified as enriched at 2-cell stage. Grey bars named Bc qPCR show fold changes between blastocyst/2-cell stages and demonstrate the tendency towards continuous decrease in the amount of these transcripts. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061547#pone-0061547-g001" target="_blank">Figure 1B</a> shows the validation results for genes identified as enriched at 4-cell stage.