Confirmation of microarray findings by qPCR analysis.

<p>qPCR analysis was performed on 7 genes to confirm the transcript abundance seen with the microarray analysis. The selected genes were <i>bmp7a</i> (A), <i>f5</i> (B)<i>, ff1d</i> (C), <i>myom1a</i> (D), <i>pomca</i> (E), star (F), <i>mc1r</i> (G). Data is presented as mean ± standard error of the mean (normalized to β-actin, SEM; n=5-7 pools of 25 embryos each); * denotes statistical significance (<i>t</i>-test, p<0.05). (See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080726#pone-0080726-t007" target="_blank">Table 7</a> for fold-changes and p-values).