Validation of microarray data and its comparison with qPCR analysis.

<p>Log ratio of microarray fold change expression of the selected 20 up and down regulated genes associated with specific biological process at 3, 6 and 18 h post PGF_2α administration in the CL. The genes selected by microarray analysis were subjected to qPCR analysis and log ratio of fold expression changes at different time points post PGF_2α administration are represented as bar graphs. Individual bar for each gene represents mean±SEM log₂ (fold change) in mRNA expression value for microarray analysis and qPCR analysis at each time point (n = 3 animals/time point). For each gene, bars with different alphabets above them are significantly different (p<0.05).