Validation of microarray data by qPCR.
- Zhibo Hou (349101)
- Chunhua Xu (391733)
- Haiyan Xie (633463)
- Huae Xu (633464)
- Ping Zhan (327107)
- Like Yu (139446)
- Xuefeng Fang (141303)
Publication date
September 2014
DOIAbstract
<p>The differential expression of 5 lncRNAs (A) or 5 mRNAs (B) in samples of 10 patients by microarray was validated by qPCR. The relative expression level in PR samples was normalized by the PD samples. The heights of the columns in the chart represent the median fold changes (PR/PD) in expression across the patients for each of the validated lncRNAs or mRNAs. Fold changes were calculated by the 2<sup>−ΔCt</sup> method. Fold changes = mean2<sup>−ΔCt</sup> (PR)/mean2<sup>−ΔCt</sup> (PD), where ΔCt = Ct<sub>target</sub>−Ct<sub>β-actin</sub>.</p
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