QRTPCR verification of selected microarray gene expression responses.

<p>The same RNA used for Agilent microarrays was examined by QRTPCR. All fold changes were calculated relative to controls. Fold changes of the 22 most differentially expressed genes in the microarray are compared with fold changes from qRT-PCR verification (<b>A</b>). Three represented genes shows correlation of the fold changes between microarray and qRT-PCR verification (<b>B</b>); bars (left y-axis) and lines (right y-axis) represent the fold-change values relative to <i>Hprt</i> for QRTPCR and microarray data, respectively; “a” indicates a statistical difference from <i>2αΔ/Δ</i>, “b” indicates a statistical difference from <i>1αΔ/Δ</i>, “c” indicates a statistical difference from <i>1/2αΔ/Δ</i>. Statistical significance indicates a p-value ≤ 0.05.