qRT-PCR validation of gene expression changes.

<p>Gene expression was measured in blood cell RNA using qRT-PCR to validate the microarray measurements. Two consistently up regulated genes are shown here, <i>IL1R1</i> (A) and <i>LY96</i> (B); and two downregulated genes, <i>MARCKSL1</i> (C) and <i>FAIM3</i> (D). Relative expression are mean fold changes at 2, 5 and 30 days in at least three biological replicates. Microarray changes are solid symbols (●) and qRT-PCR fold changes are shown in open symbols (o), representing the mean, error bars are SEM.