Validation of microarray data with qRT-PCR.

<p>(A) Quantitative real time PCR was used to quantify gene expression of selected genes from neutrophils isolates from blood and oral rinse. Results are expressed as fold vs. Blood expression used as internal control. Gene expression was normalized with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a reference gene. (B) Correspondence between mean fold change (FC) values obtained by microarray (X) vs. qRT-PCR (y) analysis. The diagonal line represents the ideal correspondence trend (Pearson correlation r = 0.8712, p-value ≤0.01). All data are mean ± SEM from 3 independent experiments (n = 4).