Validation of microarray data by quantitative real-time PCR.

<p>Six genes were selected and their time-course expression profiles were evaluated by quantitative real-time PCR in samples with (+) or without (−) DEX at given time points. (A) Fold changes were obtained from microarray analysis. (B) Fold changes were obtained from qRT-PCR analysis. Relative expression was obtained using 26S rRNA as an internal control. cDNAs were synthesised from three biological replicates.