Validation of microarray data by quantitative real-time PCR.

<p>Quantitative real-time PCR was performed for IL-8, ID2, SOX4 and SOX9. Fold-changes represent expression level of genes in isolated islets relative to intact islets. Mean fold changes derived from quantitative PCR (white bars) were plotted along with the corresponding mean fold changes from microarray analysis (solid bars). Error bars represent standard deviation. * represents t-test p-values<0.05. For microarray standard deviation and t-test was performed on the expression values obtained after normalization with Lumi. The mean fold changes determined by quantitative PCR were in concordance with the mean fold changes from microarray data.