Validation of gene array data by qRT-PCR.

<p>(A) Twenty five genes from NF-κB, cytokine, and inflammatory pathways were randomly selected and their relative mRNA levels in mock and 4OHT-treated vector and K13-ER^TAM-expressing BCBL1 cells were examined using qRT-PCR. Real-time PCR reactions were performed in triplicate and the data presented as fold change mean ±S.E in target gene expression (*p<0.05; Student's t-test). (B) Pearson Correlation coefficient between gene expression array and real time PCR showed a significant agreement (Correlation coefficient 0.88; p<0.0001).