<p>For each gene, 3 comparisons are shown in the figure. Upper panel: fold changes between RNA-Seq RPKM value; middle panel: fold changes by qRT-PCR measured in the same subjects used for RNA-Seq analysis only; lower panel: fold changes by qRT-PCR in all subjects.</p
<p>The same RNA used for Agilent microarrays was examined by QRTPCR. All fold changes were calculate...
<p>Twenty genes were chosen for RT-qPCR validation. The white and black bars represent the relative ...
Data file consists of two excel sheets. The first sheet labelled 'ct' contains all raw Ct values tha...
<p>Bar plots and inset scatter plot show a comparison of RNA-seq and RT-qPCR fold change values obta...
<p>Fold changes were calculated for 42 DEGs and a high correlation (R<sup>2</sup> > 0.93) was observ...
<p>White bars represent RNA-seq data, while the black bars represent mean values of log<sub>2</sub> ...
<p>Expression levels of 26 randomly selected genes in the four samples used in this study were detec...
<p>(a) Comparisons of changes in miRNAs targets involved in the KEGG pathway. (b) Fold changes of ge...
a<p>values based on RPKM normalization;</p>b<p>one qRT-PCR probe used;</p>c<p>not significant.</p
Five genes were selected to verify RNA-Seq results using qRT-PCR. Graphs on the left (A, C, E, G, I,...
<p>qRT-PCR validation of gene expression changes, expressed as fold-change (FC) relative to 0 mM Se....
1<p>fold change by real-time RT-PCR is relative gene expression level (chGH/PBS).</p>2<p>fold change...
<p>The black bars represent RNA-seq data, and the white bars represent the qRT-PCR data. The RNA-seq...
<p>Data were analyzed by the 2<sup>–ΔΔCt</sup> method using GAPDH as a reference gene. The results a...
<p>Note: Fold change between contrasts presented in third and fourth column. + values indicate highe...
<p>The same RNA used for Agilent microarrays was examined by QRTPCR. All fold changes were calculate...
<p>Twenty genes were chosen for RT-qPCR validation. The white and black bars represent the relative ...
Data file consists of two excel sheets. The first sheet labelled 'ct' contains all raw Ct values tha...
<p>Bar plots and inset scatter plot show a comparison of RNA-seq and RT-qPCR fold change values obta...
<p>Fold changes were calculated for 42 DEGs and a high correlation (R<sup>2</sup> > 0.93) was observ...
<p>White bars represent RNA-seq data, while the black bars represent mean values of log<sub>2</sub> ...
<p>Expression levels of 26 randomly selected genes in the four samples used in this study were detec...
<p>(a) Comparisons of changes in miRNAs targets involved in the KEGG pathway. (b) Fold changes of ge...
a<p>values based on RPKM normalization;</p>b<p>one qRT-PCR probe used;</p>c<p>not significant.</p
Five genes were selected to verify RNA-Seq results using qRT-PCR. Graphs on the left (A, C, E, G, I,...
<p>qRT-PCR validation of gene expression changes, expressed as fold-change (FC) relative to 0 mM Se....
1<p>fold change by real-time RT-PCR is relative gene expression level (chGH/PBS).</p>2<p>fold change...
<p>The black bars represent RNA-seq data, and the white bars represent the qRT-PCR data. The RNA-seq...
<p>Data were analyzed by the 2<sup>–ΔΔCt</sup> method using GAPDH as a reference gene. The results a...
<p>Note: Fold change between contrasts presented in third and fourth column. + values indicate highe...
<p>The same RNA used for Agilent microarrays was examined by QRTPCR. All fold changes were calculate...
<p>Twenty genes were chosen for RT-qPCR validation. The white and black bars represent the relative ...
Data file consists of two excel sheets. The first sheet labelled 'ct' contains all raw Ct values tha...
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