Validation of RNA-Seq through qPCR.
- Hormat Shadgou Rhein (14636561)
- Avinash Sreedasyam (246203)
- Peter Cooke (354593)
- Ciro Velasco-Cruz (6543569)
- Jane Grimwood (172021)
- Jeremy Schmutz (172027)
- Jerry Jenkins (172005)
- Sajal Kumar (14636564)
- Mingzhou Song (252550)
- Richard J. Heerema (12373438)
- L. J. Grauke (10018670)
- Jennifer J. Randall (8375826)
Publication date
February 2023
DOI Publisher
Public Library of Science (PLoS)Abstract
Five genes were selected to verify RNA-Seq results using qRT-PCR. Graphs on the left (A, C, E, G, I, K) are 2^ddCQ values from qPCR and graphs on the right (B, D, F, H, J, L) show normalized gene expression (RPKM) values from RNA-Seq. Pearson correlations analyzes was performed to determine correlation between RNA-Seq normalized gene expression (RPKM) and qPCR (2^ddCQ).</p
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