Validation of the RNA-Seq using the qRT-PCR.

Validation of RNA-seq results by qRT-PCR.

Shriram N. Rajpathak (578635)
Shamsudheen Karuthedath Vellarikkal (578636)
Ashok Patowary (108010)
Vinod Scaria (19350)
Sridhar Sivasubbu (67797)
Deepti D. Deobagkar (578637)

June 2014

In total, five genes including XIST (lincRNA) were selected for real time data validation. Relati...

Comparison of 13 differentially expressed genes from RNASeq and qRT- PCR.

Yanhong Xiao (839890)
Yonghua Wu (839891)
Keping Sun (443670)
Hui Wang (30400)
Bing Zhang (129)
Shuhui Song (36514)
Zhenglin Du (416307)
Tinglei Jiang (443673)
Limin Shi (839892)
Lei Wang (6656)
Aiqing Lin (839893)
Xinke Yue (771470)
Chenji Li (839894)
Tingting Chen (111216)
Jiang Feng (286772)

January 2016

(A) 13 genes expression from qRT-PCR. Results respresent mean + S.E.M. (N = 5) (B) 13 genes expre...

A comparison of the results from RNA-seq and from qRT-PCR analyses of differentially expressed genes.

Shanyi Chen (552080)
Ting Ye (346594)
Lu Hao (552081)
Hui Chen (28597)
Shaojie Wang (552082)
Zaifeng Fan (552083)
Liyun Guo (552084)
Tao Zhou (117050)

April 2014

A comparison of the results from RNA-seq and from qRT-PCR analyses of differentially expressed ge...

Validation of long non-coding RNAs expression patterns obtained by RNA sequencing (RNA-seq) using quantitative reverse transcription PCR (qRT-PCR).

Xiaohui Li (73588)
Xuexia Xing (5963120)
Shixiao Xu (3617453)
Mingzhen Zhang (1735672)
Yuan Wang (14955)
Hengyan Wu (5963123)
Zhihao Sun (284118)
Zhaoguang Huo (5963126)
Fang Chen (34476)
Tiezhao Yang (5963129)

November 2018

(A) Validation of RNA-seq results using qRT-PCR. (B) Correlation analysis of RNA-seq and qRT-PCR res...

Validation of RNA-Seq through qPCR.

Hormat Shadgou Rhein (14636561)
Avinash Sreedasyam (246203)
Peter Cooke (354593)
Ciro Velasco-Cruz (6543569)
Jane Grimwood (172021)
Jeremy Schmutz (172027)
Jerry Jenkins (172005)
Sajal Kumar (14636564)
Mingzhou Song (252550)
Richard J. Heerema (12373438)
L. J. Grauke (10018670)
Jennifer J. Randall (8375826)

February 2023

Five genes were selected to verify RNA-Seq results using qRT-PCR. Graphs on the left (A, C, E, G, I,...

Validation of the RNA-seq approach using q-RT-PCR.

Jeremie Vidal-Dupiol (395166)
Nolwenn M. Dheilly (165816)
Rodolfo Rondon (635431)
Christoph Grunau (269384)
Céline Cosseau (269408)
Kristina M. Smith (250856)
Michael Freitag (50158)
Mehdi Adjeroud (133233)
Guillaume Mitta (107528)

September 2014

Twenty-two genes were arbitrary selected, from highly up-regulated to highly down-regulated conti...

Correlation between RNA-Seq and qRT-PCR data of selected genes from the list of the up regulated transcripts.

Lucy Mackenzie-Impoinvil (6278108)
Gareth D. Weedall (215713)
Juan C. Lol (6278111)
Jesús Pinto (6278114)
Lucrecia Vizcaino (6278117)
Nicole Dzuris (6278120)
Jacob Riveron (3440567)
Norma Padilla (44896)
Charles Wondji (3419594)
Audrey Lenhart (354002)

January 2019

Correlation between RNA-Seq and qRT-PCR data of selected genes from the list of the up regulated tra...

Validation of the accuracy of RNA-seq data.

Tieshan Xu (513506)
Lihong Gu (513505)
Kyle Michael Schachtschneider (635715)
Xiaolin Liu (403100)
Wei Huang (36889)
Ming Xie (420493)
Shuisheng Hou (513508)

September 2014

Note: The validation of the accuracy of RNA-seq data was carried out by comparing of RPKM and the...

Correlation between RNA-Seq and qRT-PCR data of selected genes.

Yujie Mao (696356)
Lidan Xiong (696357)
Siyu Wang (696358)
Jianqiao Zhong (696359)
Rongying Zhou (696360)
Li Li (14993)

February 2015

Pearson correlation coefficient(r = 0.960) was used to determine the similarity in gene expressio...

Validation of RNA-Seq data by qRT-PCR.

Sara Torre (659944)
Massimiliano Tattini (659945)
Cecilia Brunetti (659946)
Lucia Guidi (700275)
Antonella Gori (2902535)
Cristina Marzano (34213)
Marco Landi (2902536)
Federico Sebastiani (70931)

August 2016

Comparison of expression level of nine DEGs between RNA-Seq data and qRT-PCR assay. PCR primers a...

qRT-PCR validation of the DEGs selected from the four conditions (10T2h, 50T2h, 10T24h and 50T24h).

Yongchun Shi (6725834)
Hui Sun (107933)
Xiaoran Wang (403115)
Weihuan Jin (6725837)
Qianyi Chen (6725840)
Zhengdong Yuan (6725843)
Haidong Yu (101810)

May 2019

The expression profiles were compared with the corresponding DEGs in RNA-seq data (R2 = 0.792, R2 = ...

Comparison of gene expression ratios that were obtained by RNA-seq and by quantitative real-time PCR (qRT-PCR): (a) the comparison of the gene expression value gained by RNA-seq and by qRT-PCR; (b) the liner regression analysis between the gene expression ratios obtained by RNA-seq and by qRT-PCR.

Ya-Jian Zhang (680794)
Xing-Jian Wang (680795)
Ju-Xun Wu (680796)
Shan-Yan Chen (680797)
Hong Chen (108084)
Li-Jun Chai (680798)
Hua-Lin Yi (680799)

December 2014

The black bars represent RNA-seq data, and the white bars represent the qRT-PCR data. The RNA-seq...

Validation of RNA-Seq results was performed by qRT-PCR analysis on 5 randomly chosen genes from the list of differentially expressed genes.

Olga Bajenova (3095223)
Anna Gorbunova (3095211)
Igor Evsyukov (3095229)
Michael Rayko (3095220)
Svetlana Gapon (3095217)
Ekaterina Bozhokina (3095214)
Alexander Shishkin (3095226)
Stephen J. O’Brien (108646)

September 2016

CEACAM5 also was analysed as positive control. Results obtained by RNA-Seq and qRT-PCR methods sh...

Validation of the RNAseq approach using q-RT-PCR.

Jeremie Vidal-Dupiol (395166)
Didier Zoccola (395167)
Eric Tambutté (219453)
Christoph Grunau (269384)
Céline Cosseau (269408)
Kristina M. Smith (250856)
Michael Freitag (50158)
Nolwenn M. Dheilly (165816)
Denis Allemand (213146)
Sylvie Tambutté (219459)

March 2013

Twenty-five genes that whose expression was significantly different in the control and treatment ...

Correlation between qRT-PCR and RNA-Seq data.

David W. Y. Ho (164778)
Zhen Fan Yang (164785)
Kang Yi (164792)
Chi Tat Lam (164799)
Michael N. P. Ng (164805)
Wan Ching Yu (164810)
Joyce Lau (164816)
Timothy Wan (164820)
Xiaoqi Wang (164823)
Zhixiang Yan (164824)
Hang Liu (3694)
Yong Zhang (5893)
Sheung Tat Fan (164825)

May 2012

Correlation between qRT-PCR and RNA-Seq data of 47 selected genes: 28 up-regulated genes and 19 d...

Validation of RNA-seq results by qRT-PCR.

Shriram N. Rajpathak (578635)
Shamsudheen Karuthedath Vellarikkal (578636)
Ashok Patowary (108010)
Vinod Scaria (19350)
Sridhar Sivasubbu (67797)
Deepti D. Deobagkar (578637)

June 2014

In total, five genes including XIST (lincRNA) were selected for real time data validation. Relati...

Comparison of 13 differentially expressed genes from RNASeq and qRT- PCR.

Yanhong Xiao (839890)
Yonghua Wu (839891)
Keping Sun (443670)
Hui Wang (30400)
Bing Zhang (129)
Shuhui Song (36514)
Zhenglin Du (416307)
Tinglei Jiang (443673)
Limin Shi (839892)
Lei Wang (6656)
Aiqing Lin (839893)
Xinke Yue (771470)
Chenji Li (839894)
Tingting Chen (111216)
Jiang Feng (286772)

January 2016

(A) 13 genes expression from qRT-PCR. Results respresent mean + S.E.M. (N = 5) (B) 13 genes expre...

A comparison of the results from RNA-seq and from qRT-PCR analyses of differentially expressed genes.

Shanyi Chen (552080)
Ting Ye (346594)
Lu Hao (552081)
Hui Chen (28597)
Shaojie Wang (552082)
Zaifeng Fan (552083)
Liyun Guo (552084)
Tao Zhou (117050)

April 2014

A comparison of the results from RNA-seq and from qRT-PCR analyses of differentially expressed ge...

Validation of long non-coding RNAs expression patterns obtained by RNA sequencing (RNA-seq) using quantitative reverse transcription PCR (qRT-PCR).

Xiaohui Li (73588)
Xuexia Xing (5963120)
Shixiao Xu (3617453)
Mingzhen Zhang (1735672)
Yuan Wang (14955)
Hengyan Wu (5963123)
Zhihao Sun (284118)
Zhaoguang Huo (5963126)
Fang Chen (34476)
Tiezhao Yang (5963129)

November 2018

(A) Validation of RNA-seq results using qRT-PCR. (B) Correlation analysis of RNA-seq and qRT-PCR res...

Validation of RNA-Seq through qPCR.

Hormat Shadgou Rhein (14636561)
Avinash Sreedasyam (246203)
Peter Cooke (354593)
Ciro Velasco-Cruz (6543569)
Jane Grimwood (172021)
Jeremy Schmutz (172027)
Jerry Jenkins (172005)
Sajal Kumar (14636564)
Mingzhou Song (252550)
Richard J. Heerema (12373438)
L. J. Grauke (10018670)
Jennifer J. Randall (8375826)

February 2023

Five genes were selected to verify RNA-Seq results using qRT-PCR. Graphs on the left (A, C, E, G, I,...

Validation of the RNA-seq approach using q-RT-PCR.

Jeremie Vidal-Dupiol (395166)
Nolwenn M. Dheilly (165816)
Rodolfo Rondon (635431)
Christoph Grunau (269384)
Céline Cosseau (269408)
Kristina M. Smith (250856)
Michael Freitag (50158)
Mehdi Adjeroud (133233)
Guillaume Mitta (107528)

September 2014

Twenty-two genes were arbitrary selected, from highly up-regulated to highly down-regulated conti...

Correlation between RNA-Seq and qRT-PCR data of selected genes from the list of the up regulated transcripts.

Lucy Mackenzie-Impoinvil (6278108)
Gareth D. Weedall (215713)
Juan C. Lol (6278111)
Jesús Pinto (6278114)
Lucrecia Vizcaino (6278117)
Nicole Dzuris (6278120)
Jacob Riveron (3440567)
Norma Padilla (44896)
Charles Wondji (3419594)
Audrey Lenhart (354002)

January 2019

Correlation between RNA-Seq and qRT-PCR data of selected genes from the list of the up regulated tra...

Validation of the accuracy of RNA-seq data.

Tieshan Xu (513506)
Lihong Gu (513505)
Kyle Michael Schachtschneider (635715)
Xiaolin Liu (403100)
Wei Huang (36889)
Ming Xie (420493)
Shuisheng Hou (513508)

September 2014

Note: The validation of the accuracy of RNA-seq data was carried out by comparing of RPKM and the...

Correlation between RNA-Seq and qRT-PCR data of selected genes.

Yujie Mao (696356)
Lidan Xiong (696357)
Siyu Wang (696358)
Jianqiao Zhong (696359)
Rongying Zhou (696360)
Li Li (14993)

February 2015

Pearson correlation coefficient(r = 0.960) was used to determine the similarity in gene expressio...

Validation of RNA-Seq data by qRT-PCR.

Sara Torre (659944)
Massimiliano Tattini (659945)
Cecilia Brunetti (659946)
Lucia Guidi (700275)
Antonella Gori (2902535)
Cristina Marzano (34213)
Marco Landi (2902536)
Federico Sebastiani (70931)

August 2016

Comparison of expression level of nine DEGs between RNA-Seq data and qRT-PCR assay. PCR primers a...

qRT-PCR validation of the DEGs selected from the four conditions (10T2h, 50T2h, 10T24h and 50T24h).

Yongchun Shi (6725834)
Hui Sun (107933)
Xiaoran Wang (403115)
Weihuan Jin (6725837)
Qianyi Chen (6725840)
Zhengdong Yuan (6725843)
Haidong Yu (101810)

May 2019

The expression profiles were compared with the corresponding DEGs in RNA-seq data (R2 = 0.792, R2 = ...

Comparison of gene expression ratios that were obtained by RNA-seq and by quantitative real-time PCR (qRT-PCR): (a) the comparison of the gene expression value gained by RNA-seq and by qRT-PCR; (b) the liner regression analysis between the gene expression ratios obtained by RNA-seq and by qRT-PCR.

Ya-Jian Zhang (680794)
Xing-Jian Wang (680795)
Ju-Xun Wu (680796)
Shan-Yan Chen (680797)
Hong Chen (108084)
Li-Jun Chai (680798)
Hua-Lin Yi (680799)

December 2014

The black bars represent RNA-seq data, and the white bars represent the qRT-PCR data. The RNA-seq...

Validation of RNA-Seq results was performed by qRT-PCR analysis on 5 randomly chosen genes from the list of differentially expressed genes.

Olga Bajenova (3095223)
Anna Gorbunova (3095211)
Igor Evsyukov (3095229)
Michael Rayko (3095220)
Svetlana Gapon (3095217)
Ekaterina Bozhokina (3095214)
Alexander Shishkin (3095226)
Stephen J. O’Brien (108646)

September 2016

CEACAM5 also was analysed as positive control. Results obtained by RNA-Seq and qRT-PCR methods sh...

Validation of the RNAseq approach using q-RT-PCR.

Jeremie Vidal-Dupiol (395166)
Didier Zoccola (395167)
Eric Tambutté (219453)
Christoph Grunau (269384)
Céline Cosseau (269408)
Kristina M. Smith (250856)
Michael Freitag (50158)
Nolwenn M. Dheilly (165816)
Denis Allemand (213146)
Sylvie Tambutté (219459)

March 2013

Twenty-five genes that whose expression was significantly different in the control and treatment ...

Correlation between qRT-PCR and RNA-Seq data.

David W. Y. Ho (164778)
Zhen Fan Yang (164785)
Kang Yi (164792)
Chi Tat Lam (164799)
Michael N. P. Ng (164805)
Wan Ching Yu (164810)
Joyce Lau (164816)
Timothy Wan (164820)
Xiaoqi Wang (164823)
Zhixiang Yan (164824)
Hang Liu (3694)
Yong Zhang (5893)
Sheung Tat Fan (164825)

May 2012

Correlation between qRT-PCR and RNA-Seq data of 47 selected genes: 28 up-regulated genes and 19 d...

Validation of RNA-seq results by qRT-PCR.

Shriram N. Rajpathak (578635)
Shamsudheen Karuthedath Vellarikkal (578636)
Ashok Patowary (108010)
Vinod Scaria (19350)
Sridhar Sivasubbu (67797)
Deepti D. Deobagkar (578637)

June 2014

In total, five genes including XIST (lincRNA) were selected for real time data validation. Relati...

Comparison of 13 differentially expressed genes from RNASeq and qRT- PCR.

Yanhong Xiao (839890)
Yonghua Wu (839891)
Keping Sun (443670)
Hui Wang (30400)
Bing Zhang (129)
Shuhui Song (36514)
Zhenglin Du (416307)
Tinglei Jiang (443673)
Limin Shi (839892)
Lei Wang (6656)
Aiqing Lin (839893)
Xinke Yue (771470)
Chenji Li (839894)
Tingting Chen (111216)
Jiang Feng (286772)

January 2016

(A) 13 genes expression from qRT-PCR. Results respresent mean + S.E.M. (N = 5) (B) 13 genes expre...

A comparison of the results from RNA-seq and from qRT-PCR analyses of differentially expressed genes.

Shanyi Chen (552080)
Ting Ye (346594)
Lu Hao (552081)
Hui Chen (28597)
Shaojie Wang (552082)
Zaifeng Fan (552083)
Liyun Guo (552084)
Tao Zhou (117050)

April 2014

A comparison of the results from RNA-seq and from qRT-PCR analyses of differentially expressed ge...

Validation of the RNA-Seq using the qRT-PCR.

Abstract

Extracted data

Validation of the RNA-Seq using the qRT-PCR.

Abstract

Extracted data

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