Validation of RNA sequencing findings using qPCR.

<p>The same RNA used for RNAseq was used for qPCR. The ΔΔCt method was used for calculation of gene expression changes and actin was used as an internal control. Data analysis/grouping was performed in a similar way as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0169671#pone.0169671.g003" target="_blank">Fig 3</a>. Three of the most up-regulated and down-regulated genes of DEG groups 3, 4, and 5 were studied and compared to RNAseq results. Values of Log₂(Fold of change): >0: up-regulation; = 0 non change; <0 down-regulation. n = 3.