<p>Gene specific primers for RT-qPCR were mentioned in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114372#pone.0114372.s006" target="_blank">Table S2</a>. The detailes of PCR condition for each target gene were descrived in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114372#pone.0114372.s012" target="_blank">Supporting Information S1</a>. (A)–(D): Bar graphs showing gene expression relative to <i>gapdh</i> (means; bars = S.D). MH: adult male head (n = 3), MA: adult male abdomen (n = 3), FH: adult female head (n = 3), FA; adult female abdomen (n = 3), cleavage: cleavage-stage embryo (1–8 h post-oviposition, n = 9), morula: primary morula-stage embryo (12–24 h post-oviposition, n = 12). ...
RT-qPCR was performed on the 61 genes extracted from microarray analysis. Only the 12 genes that wer...
<p>Putative functional role is based on <a href="http://www.ncbi.nlm.nih.gov/protein" target="_blank...
<p>The genes are shown with functional information extracted from Flybase. Expression changes (log2 ...
<p>Gene specific primers for RT-qPCR were mentioned in <a href="http://www.plosone.org/article/info:...
<p>Agarose gel electrophoresis of the RT-PCR products using primers (<a href="http://www.plosone.org...
<p>Note: RT primers were used for retrotranscription reaction. Forward (F) and Reverse (R) primers w...
Sequences of primers used for RT-qPCR to assess the expression of the indicated genes.</p
<p>E: Efficiency assay using fetal and adult hippocampus pooled RNA. a: <a href="http://www.plosone....
Quantitative reverse transcriptase PCR (RT-qPCR), a powerful and efficient means of rapidly comparin...
<p>The primers target genes for signal transduction, stress response, xenobiotic metabolism, JH bios...
<p>Graphs showing fold changes between 4-cell/2-cell stages derived from ddCt values (black bars; qP...
<p>* Commonly used reference genes.</p><p>RT-qPCR primer sets used in this study and their target ge...
<p>Sequence of primers used in the qPCR process is detailed in <a href="http://www.plosone.org/artic...
Reverse-transcriptase quantitative Polymerase Chain Reaction (RT-qPCR) is a well-established techniq...
<p>The primers used for RT-qPCR analysis of the genes assessed in this study, including the gene sym...
RT-qPCR was performed on the 61 genes extracted from microarray analysis. Only the 12 genes that wer...
<p>Putative functional role is based on <a href="http://www.ncbi.nlm.nih.gov/protein" target="_blank...
<p>The genes are shown with functional information extracted from Flybase. Expression changes (log2 ...
<p>Gene specific primers for RT-qPCR were mentioned in <a href="http://www.plosone.org/article/info:...
<p>Agarose gel electrophoresis of the RT-PCR products using primers (<a href="http://www.plosone.org...
<p>Note: RT primers were used for retrotranscription reaction. Forward (F) and Reverse (R) primers w...
Sequences of primers used for RT-qPCR to assess the expression of the indicated genes.</p
<p>E: Efficiency assay using fetal and adult hippocampus pooled RNA. a: <a href="http://www.plosone....
Quantitative reverse transcriptase PCR (RT-qPCR), a powerful and efficient means of rapidly comparin...
<p>The primers target genes for signal transduction, stress response, xenobiotic metabolism, JH bios...
<p>Graphs showing fold changes between 4-cell/2-cell stages derived from ddCt values (black bars; qP...
<p>* Commonly used reference genes.</p><p>RT-qPCR primer sets used in this study and their target ge...
<p>Sequence of primers used in the qPCR process is detailed in <a href="http://www.plosone.org/artic...
Reverse-transcriptase quantitative Polymerase Chain Reaction (RT-qPCR) is a well-established techniq...
<p>The primers used for RT-qPCR analysis of the genes assessed in this study, including the gene sym...
RT-qPCR was performed on the 61 genes extracted from microarray analysis. Only the 12 genes that wer...
<p>Putative functional role is based on <a href="http://www.ncbi.nlm.nih.gov/protein" target="_blank...
<p>The genes are shown with functional information extracted from Flybase. Expression changes (log2 ...