Inhibition of JNK reduced axonal elongation on hippocampal neurons treated with CGZ.

<p>Cultured hippocampal neurons were treated with CGZ (10 µM) and CGZ + SP (CGZ, 10 µM + SP, 100 nM) for 24 (<b>A</b>), 48 (<b>B</b>), and 72 (<b>C</b>) h. Representative confocal images labeled with anti-tau 1 antibody were evaluated by the Sholl analysis. Black brackets indicate significant differences between treatment with CGZ and untreated neurons (C), blue brackets indicate significant differences between treatment with CGZ and CGZ plus SP. Data are the mean ± S.E.M. of 4 independent experiments. *p<0.05.