Hippocampal neurons are inhibited by CSPGs, and rescued by GPX3 overexpression.

<p>Hippocampal neurons transfected with active genes were plated on CSPG or laminin substrates. <b><i>A</i></b>, CSPGs (white) strongly inhibited hippocampal growth compared to laminin (black) (p<0.0001, Mann Whitney U Test) in three independent experiments (triangle, square, and circle markers). Horizontal bar indicates the average neurite total length on CSPGs and laminin, 15.5 µm and 60.9 µm respectively. <b><i>B,C</i></b> Mean Z-Scores of transfected neurons with standard deviations, centered on the negative control, mCherry. Asterisks indicate significant effects (*, **, ***, p<0.05, 0.01, 0.001 Tukey-Kramer) compared to the mCherry control when analyzed with ANOVA (p<0.001). <b><i>D, E</i></b>, Representative images of hippocampal neurons growing on CSPGs transfected with GPX3 (<b>D</b>) or control mCherry (<b>E</b>). Scale bar 100 µm.