Differential effects of GSK3 inhibition on synaptic density.

<p><b>A.</b> Example of a 21 DIV hippocampal neuron in culture. Scale bar = 10 μm. Bottom pictures: detailed magnification of the area depicted by the white box. Synapses (a) were localized following a double criterion: puncta positive for Synapsin (b, red) and for Bassoon (c, green). Scale bar 3 μm. <b>B.</b> Plot graph of the average changes in synaptic density at 21 DIV after AR-A014418 treatment. PTD4-PI3KAc and PTD4 were used at 21 μM. <b>C.</b> Plot graph of the results of synaptic density after the GSK3 inhibition by SB 415286 treatment. <b>D.</b> Quantification of synaptic density at 12 DIV after treatments with PTD4-PI3KAc, AR-A014418, SB 415286 alone or in combination with PTD4-PI3KAc. Percentage of change refers to PTD4 control treatment. Cultures were treated for 48 hours before fixation (n = 10–12 from 3 different cultures). Significance refers to the statistical analysis of each experimental condition versus PTD4 treated cultures (100%) Student’s two-tailed t-test, *p<0.05, **p<0.005, ***p<0.0001.