aNogo66 mAb and aNogoA-N mAb enhanced the axon growth of hippocampal neurons by blocking NogoA inhibition.

<p><b>A</b>: Dissociated rat E18.5 hippocampal neurons were cultured on the control substrate PLL (control group). <b>B, D</b>: Hippocampal neurons cultured on 100 pmol NogoA FC (aa 1026–1090) or NogoA FC (aa 544–725) exhibited strongly inhibited axon growth. <b>C, E</b>: Hippocampal neurons were cultured on NogoA FC (aa 1026–1090) and treated with aNogo66 mAb, or neurons were cultured on NogoA FC (aa 544–725) and treated with aNogoA-N mAb, to assess the contribution of the two mAbs on inhibition. <b>F</b>: Statistical analysis of hippocampal neuron axon growth in each group is expressed as the mean ± SEM of each group in each separate experiment (^##<i>P</i><0.01, b group or d group vs. a group; ^**<i>P</i><0.01, c group vs. b group and e group vs. d group. n = 6 wells per condition; scale bars = 100 µm).