Wnt 5A and TGZ increases axonal elongation through JNK pathway.

<p>(<b>A</b>) Representative confocal images of hippocampal neurons treated with Wnt 5A conditioned medium and 10 µM TGZ in the presence or absence of the JNK inhibitor SP600125 (SP, 100 nM) for 72 h. After treatment neurons were fixed and immunolabeled with anti-tau-1 and anti-p-JNK antibodies. Treatment with Wnt 5A and TGZ significantly increased axon growth. SP prevented axonal elongation induced by Wnt 5A and TGZ. Inhibition of JNK by SP prevented the increase of p-JNK level induced by Wnt 5A and TGZ, respectively. Representative images were taken from 4 independent experiments. (<b>B</b>) Quantification of axonal length on hippocampal neurons treated with Wnt 5A and TGZ in the presence or absence of SP for 72 h. Data are the mean ± S.E.M. of 4 independent experiments. *<i>p</i><0.05 compared with untreated neurons, ** <i>p</i><0.05 compared with Wnt 5A treated neurons, *** <i>p</i><0.05 compared with Wnt 5A+ TGZ treated neurons.