ProBDNF treatment decreases neurite length on DRG or cortical neurons.

<p>A, Time-lapse recordings show the collapsed neurite growth with proBDNF (left), and enhanced neurite growth with BDNF (right). Frames indicate 5, 10, 20, 30 min respectively. Scale bar, 20 µm. B, Treatment of DRG neurons with proBDNF decreased the rate of neurite extension within 3 min and remained decelerated through 30 min. <i>n</i> = 30 neurites/treatment. *<i>p</i><0.05, **<i>p</i><0.001, compared to BSA; Studens <i>t</i> test. C, Treated cortical or DRG neurons with proBDNF caused similar collapse in neurite length. <i>n</i> = 83–85 neurons/treatment. *<i>p</i><0.05, **<i>p</i><0.001, significantly different from untreated neurons; Studens <i>t</i> test. D, Expression of sortilin, p75NTR on the lysate of cultured cortical or DRG neurons processed for Western blot. Bands of 35 kDa of proBDNF, 75 kDa of p75NTR, and 110 kDa of sortilin were detected with their respective antibodies. ß-actin (42 kD) antibody was used as internal protein loading control. <i>n</i> = 3 independent experiments.