SnoN Promotes Neurite Outgrowth From Adult DRG Cultures.

<p>(<b>A</b>) Western blot from HEK293 cells electroporated to express GFP, wild-type SnoN, SnoN-DBM, GFP fused SnoN, and GFP fused SnoN-DBM. Cells transfected with wild-type SnoN and SnoN-DBM demonstrate SnoN immunoreactive bands at ~75 kD (lane 3 and 4). Cells transfected with GFP-SnoN and GFP-SnoN-DBM demonstrate SnoN immunoreactive bands and corresponding GFP immunoreactive bands at ~110 kD, a molecular weight consistent with expression a GFP fused variant. (<b>B</b>) Neurite outgrowth from control adult DRG neurons in vitro. NF200 label. (<b>C</b>) Overexpression of SnoN increases neurite length, quantified in E. Infected cells express GFP and are labeled for NF200 (shown). (<b>D</b>) Overexpression of SnoN-D-box mutation further amplifies neurite growth. (<b>E</b>) Quantification confirms that SnoN expression in adult DRG neurons significantly enhances neurite growth (ANOVA p<0.001; post hoc Fisher’s ***p<0.001 comparing treated groups to GFP, and **p<0.01 comparing SnoN to SnoN-DBM group).