DRG neurons co-cultured with cells of the four groups.

<p>(A–E) Immunocytochemical staining for βIII-tubulin (FITC) to show neurites sprouting from DRG neurons after co-culture for 24 h with basal media (control, A), undifferentiated BMSCs (B), SC-like cells cultured in conventional differentiate media (C), SC-like cells cultured in optimal differentiate media (D) and Schwann cells (E). Scale bar: 100 µm. (F–H) Three independent parameters measured: percentage of DRG neurons sprouting neurites (F), length of longest neurite (G) and total neurite density (H). Data were listed as mean ± S.E.M. Control vs. group A and basal media: *<i>p</i><0.05, **<i>p</i><0.01; Control vs. group B: ^#<i>p</i><0.05, ^##<i>p</i><0.01.