Late passage mesenchymal stem cells enhance neurite outgrowth of co-cultured DRG neurons.

<p>(<b>A</b>) Immunocytochemical staining for βIII tubulin (Alexa Fluor 488) visualized neurite outgrowth from DRG following 24 h co-culture with medium only controls (α-MEM and diff α-MEM) and undifferentiated MSC (uMSC) and differentiated MSC (dMSC) from young and old donors (T12) seeded in tissue culture inserts above the neurons. (<b>B</b>) Quantitative analysis of total neurite outgrowth in the co-cultures. ***P<0.001 significantly different to respective media only controls. n.s not significantly different (α-MEM and diff α-MEM versus DRG alone and dMSC versus uMSC old donors).