Composition of immune infiltrate of MCA-205-OVA tumors.

<p><b>A</b>) Representative gating strategy to determine immune infiltrate of MCA-205-OVA tumors. B6 mice were administered 1×10⁵ MCA-205-OVA cells, and tumors were excised and digested when the tumor reached 15 mm. The immune cells which infiltrated the tumor were determined by flow cytometry. CD45⁺, live cells were first gated. Macrophages (CD45⁺CD11b⁺ CD11c^lo F4/80⁺GR-1⁻), myeloid-derived suppressor cells [(MDSC) CD45⁺CD11b⁺ CD11c^lo GR-1⁺], CD4 T cells (CD45⁺CD11b⁻CD3⁺CD4⁺), CD8 T cells (CD45⁺CD11b⁻CD3⁺CD8⁺), and NK cells (CD45⁺CD11b⁻CD3⁻CD8⁻CD4⁻NK1.1⁺) were quantified in <b>B</b> as the percentage of CD45⁺ cells. Tregs were <1%, not shown in figure. Data shown is the mean ± SEM from three mice.