Characterization of hematopoietic cells within the tumor microenvironment of MT/ret mice.

<p>(<b>A</b>) Dot plots from a cell suspension of one representative cutaneous metastasis. Myeloid and lymphoid stainings were performed as defined in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020235#pone-0020235-g001" target="_blank">Fig 1A</a>. (<b>B</b>) The pie diagram summarizes the proportion of hematopoietic cells from 47 cutaneous metastasis. (<b>C</b>) Variability of the proportion of tumor infiltrating CD11b⁺ cells from cutaneous metastasis. The frequencies of CD11b⁺ cells gated from CD45⁺ cells were determined. The graph shows the data for four 3 month old mice. (<b>D</b>) Correlation between the tumor aggressiveness and the increase of tumor infiltrating CD11b⁺ cells. The tumor aggressiveness corresponds to the ratio between the absolute numbers of tumor associated cells and the number of days since appearance of each nodule. Statistical differences were assessed using ANOVA test.