Immunization with MCA-205-OVA, MCA-205-E1A-Δp300-OVA and MCA-205-E1A-OVA tumor cells provided concomitant tumor immunity against MCA-205-OVA challenge on the contralateral flank of B6 mice.

<p>Mice were injected with 1×10⁵ live MCA-205, MCA-205-OVA, MCA-205-E1A-Δp300-OVA, or MCA-205-E1A-OVA tumor cells s.c. in the flank three times, five days apart. Five days after last tumor dose (day 20), mice were challenged with serial log dilutions of MCA-205-OVA tumor cells on the contralateral flank from 1×10¹–1×10⁶ cells. The TPD₅₀ of MCA-205-OVA was calculated six weeks later. Data shown is the mean ± SEM from three experiments, with three mice per dose per experiment. ***, p<.001.