Analysis of subsets of T cells infiltrating subcutaneous B16-F10 tumors 3 days after intratumoral virus treatment in C57BL/6 mice.

<p>C57BL/6 mice (n = 3 per group) were implanted with unilateral subcutaneous B16-F10 tumor cells. The first dose of the virus (2.6×10⁷ PFU i.t.) was given on day 7 (when tumors reached approximately 100 mm³) and the second dose was given on day 10. Treatment groups are: 1. vMyx-IL15Rα-tdTr, 2. vMyx-tdTr, 3. PBS. Mice were euthanized 3 days after the final virus treatment and tumor sections were analyzed for presence of T cells by immunostaining for CD4 and CD8 markers. Representative tumor sections are shown. (A) Staining for CD8⁺ T cells in tumors. Green – CD8-positive cells, Blue – DAPI. Scale bar = 50 micrometers. (B) Staining for CD4⁺ T cells in tumors. Red – CD4-positive cells, Blue – DAPI. Scale bar = 50 micrometers.