Effects of <i>Ano6</i>-KD on the proliferation of C2C12 myoblasts.

<p>(A) Relative expression of Ano6 (normalized to GAPDH) examined by quantitative RT-PCR in C2C12 stable cells lines (Scramble [shSCR], <i>Ano6</i>-KD). (B) Representative photographs of stable C2C12 cell lines expressing either a Scramble shRNA or the shRNA targeting <i>Ano6</i>-KD 48 hours post plating. (C) Quantitative analysis of C2C12 myoblast proliferation using the MTT assay. Scale bar = 150 μm. ***p<0.001.