Effect of C9orf116 on BRL-3A cell proliferation.

<p>(A) The cell viability was assessed at 24, 48 and 72 h after transfected with siRNA and control by MTT assay. (B) The cell viability was assessed at 24, 48 and 72 h in C9orf116 over-expression cells and control by MTT assay. (C) Proliferation measurement by counting live cells in haemocytometer chamber after trypan blue staining, the number of live cells was counted at 24, 48 and 72 h after transfected with siRNA and control. (D) The number of live cells was counted at 24, 48 and 72 h in C9orf116 over-expression cells and control. NC, C9-siR2 represents the cells treated with negative siRNA, siR2-C9orf116, respectively; pCDH, pCDH-C9 represents the cells treated with Empty vector (pCDH), pCDH-C9orf116, respectively. The above results were presented as means ± SD. The experiments were performed three times and three replications per group. * Indicates <i>p</i> <0.05.