Population doubling assay and Cell cycle analysis of BM-MSCs.

<p>(A) MSCs from Mutant, Lean and Control, were measured for proliferation rate at 24, 48, 72 and 96 hrs. BM-MSCs from Mutants showed an increase in proliferation at all the time points and at 96 hours, the PDA was significantly higher compared to Lean and Control. Values represent the absolute cell count and have been computed from three independent experiments performed in duplicates (Mean ± SE, n = 6) (B) Cell cycle analysis of BM-MSCs from Mutants showing an increase in ‘S’ phase and G2-M as compared to Lean (C) and Control (D). Values have been represented as Mean ± SE (n = 6) from three individual experiments performed in duplicates. (E) Cell cycle analysis of BM-MSCs showing an increase in BrdU incorporation (‘S’ phase) (green) from Mutant compared to Lean and Control and proliferation index (F). Values have been represented as Mean ± SE (n = 6) from three individual experiments performed in duplicates. Asteric (*) indicates p<0.05 using ANOVA.