Phenotypic assays.

<p>Horizontal brackets indicate significant differences between the specified conditions, as calculated using ANOVA (p-value<0.05). <b>A</b>. The MTT assay measures cell proliferation, with absorbance being correlated with cell growth. <b>B</b>. Doubling time was calculated from cell counts taken over 3 days (average of 3 replicates per condition). <b>C</b>. The EdU incorporation was determined via FACS, and reveals the cell cycle distribution. The graph shows the percent of cells in S phase. <b>D</b>. Relative telomerase activity is shown. <b>E</b>. Average number of telomere repeat fragments (TRF, average of 3 replicates) for each condition. <b>F</b>. Percent of apoptotic cells, determined using TUNEL-FACS. <b>G</b>. Example of an OCT4-positive focus in a teratoma section. <b>H</b>. Bar graph showing, for each culture condition, the percent of teratoma sections containing at least one OCT4-positive focus, and the average number of foci per section. The error bar indicates the standard deviation of counts from 6 (EcmEnz), 7 (MefMech), or 8 (MefEnz and EcmMech) sections. Raw counts can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0118307#pone.0118307.s007" target="_blank">S4 Table</a>).