Cytotoxic effect and proliferative effect of TCS on anoikis resistant H460 cells.

<p>(A) The chemical structure of TCS. (B) Multicellular aggregation of anoikis resistant H460 cells. Scale bar is 1,000 µm. (C–D) After treatment with TCS (0–10 µM) for 24 h, the percentage of cell viability was determined by MTT assay and the percentage of apoptotic cells was detected by Hoechst33342 staining, respectively. Values are means of the three independent triplicate samples ± SE. *<i>P</i><0.05 versus non-treated control. (E) After the indicated treatment, nuclear morphology of the cells was detected by Hoechst33342/PI co-staining assay and visualized under a fluorescence microscope. Scale bar is 50 µm. (F) Cells were treated with TCS (0–7.5 µM) for 24, 48 or 72 h. Cell viability was determined by MTT assay. The data represent the means of the three independent triplicate samples ± SE. *<i>P</i><0.05 versus non-treated control at each indicated time. (G–H) Cells were treated with TCS (0–7.5 µM) for 48 h. Cell cycle of TCS-treated cells was determined by PI staining and flow cytometry. *<i>P</i><0.05 versus non-treated control.