Effect of C6-Cer on cell proliferation.

<p>A. FRTL-5 cells were preincubated for 48 h with 0.05 mM C6-Cer/CholPC or C6-Cer/DMSO. [³H]Thymidine incorporation into cellular DNA during the last 4 h was determined. B. Cell proliferation measurement using cell count. Effect of C6-Cer on cell proliferation on FRTL-5 was measured by counting the cells after preincubation for 48 h with 0.05 mM C6-Cer/CholPC or C6-Cer/DMSO. DMSO alone was used as control. Each value gives the amount of cells per plate. C. HeLa cells were incubated with 0.05 mM C6-Cer/CholPC or C6-Cer/DMSO for 12 h. [³H]Thymidine incorporation into cellular DNA during the last 4 h was determined. D. FRTL-5 cells were exposed to 0.05 mM L-erythro-C6-Cer/CholPC or C6-dihydroCer/CholPC for 24 h after which [³H]thymidine incorporation into cellular DNA during the last 4 h was determined. E. FRTL-5 cells were exposed for 48 h to 0.05 mM Chol/CholPC, Chol/DMSO, or Chol/m-β-cyclodextrin after which [³H]thymidine incorporation into cellular DNA during the last 4 h was determined. Each value is the mean ± SEM of at least 3 independent experiments. *P<0.05; **P<0.01; ***P<0.001.