MSCs influence C2C12 myoblast proliferation through paracrine mechanisms.

<p>C2C12 cells were grown in single culture (C2C12) or exposed to MSC-derived CM (C2C12/MSC-CM) and their proliferative activity assessed by time lapse videomicroscopy (A-C), EdU (green) incorporation (D, E), Notch-1 and Hes-1 expression by RT-PCR (F), Western blotting (G) and confocal immunofluorescence (H). Quantitative analyses of the results shown are reported in the histograms. Data represent the results of at least three independent experiments with similar results. * <i>p<0.05</i> versus T0; ° <i>p<0.05</i> versus the earlier time points; § <i>p<0.05</i> vs 9 h; # <i>p<0.05</i> versus single culture. D,E: * <i>p<0.05</i>.