Representative micrographs of Scramble and <i>Ano6</i>-KD C2C12 myotubes.

<p>(A) Scramble and <i>Ano6</i>-KD stable cells, which also express mCherry, were imaged 3days after differentiation. (B) Fusion index of Scramble and <i>Ano6</i>-KD stable C2C12 cells after differentiation for 3 days. Scale bar = 150 μm. (C,D) Fold difference of myogenin and myosin heavy chain (MHC) expression as examined by RT-PCR of C2C12 cells during differentiation.