<p>Confluent mAECs were incubated with 250 µg/mL AST or ASIV for 2 h, then cell surface proteins were isolated and TNFR1 expressed on the cell surface was measured by Western blot analysis. Data were representative of three independent experiments with determinations made in triplicate and shown as mean values+S.D. **P<0.01 compared with cells treated with control group. CN: control.</p
BEAS-2Bs were exposed to EPFR (DCB230 or MCP230) at 50 μg/cm2 or 100 μg/cm2 for 24h, and TNF-α produ...
<p>(A) Splenocytes were harvested 6 h after stimulation by PA-MSHA and lysate by RIPA buffer. Expres...
<p>(A, B) Soluble protein extracts from HeLa cells transfected with pro-WT were sequentially immunop...
<p>Confluent mAECs were pre-incubated with 250 µg/mL of AST or ASIV for 2 h, then washed and incubat...
<p>Confluent mAECs were pre-incubated with 250 µg/mL of AST or ASIV for 2 h, then washed and incubat...
<p>FACS analysis was performed in HEK293T cells transiently expressing the WT and mutated forms of t...
<p>Confluent mAECs were pre-incubated with 250 µg/mL AST or ASIV for 2 h, then washed and incubated ...
<p>Confluent mAECs were pre-incubated with 250 µg/mL of AST or ASIV for 2 h, then washed and incubat...
<p>Results shown in <b>A</b> and <b>B</b> were obtained in one experiment and results shown in <b>C<...
<p>(A) The mRNA of TNFR1 (301 bp) but not TNFR2 (264 bp) was identified in OECs by RT-PCR. (B) Cultu...
<p>Western blotting assay (A) revealed that 1 uM of SIM could decrease TNFR1 expression in the absen...
<p>Confluent mAECs were pre-incubated with 250 µg/mL ASI, ASII or ASIII for 2 h, then washed and inc...
<p>All values represent means ± SE of five independent experiments. A.U.: arbitrary units.</p
<p>U937 cells were treated with LPS or ASTA at the doses indicated. Groups include the following: Nu...
<p>Cell lysates of PC14PE6/AS2-Vec(1), S3C(1), and S3C(2) (A) and cell lysates of Vec(1), S3D(1), an...
BEAS-2Bs were exposed to EPFR (DCB230 or MCP230) at 50 μg/cm2 or 100 μg/cm2 for 24h, and TNF-α produ...
<p>(A) Splenocytes were harvested 6 h after stimulation by PA-MSHA and lysate by RIPA buffer. Expres...
<p>(A, B) Soluble protein extracts from HeLa cells transfected with pro-WT were sequentially immunop...
<p>Confluent mAECs were pre-incubated with 250 µg/mL of AST or ASIV for 2 h, then washed and incubat...
<p>Confluent mAECs were pre-incubated with 250 µg/mL of AST or ASIV for 2 h, then washed and incubat...
<p>FACS analysis was performed in HEK293T cells transiently expressing the WT and mutated forms of t...
<p>Confluent mAECs were pre-incubated with 250 µg/mL AST or ASIV for 2 h, then washed and incubated ...
<p>Confluent mAECs were pre-incubated with 250 µg/mL of AST or ASIV for 2 h, then washed and incubat...
<p>Results shown in <b>A</b> and <b>B</b> were obtained in one experiment and results shown in <b>C<...
<p>(A) The mRNA of TNFR1 (301 bp) but not TNFR2 (264 bp) was identified in OECs by RT-PCR. (B) Cultu...
<p>Western blotting assay (A) revealed that 1 uM of SIM could decrease TNFR1 expression in the absen...
<p>Confluent mAECs were pre-incubated with 250 µg/mL ASI, ASII or ASIII for 2 h, then washed and inc...
<p>All values represent means ± SE of five independent experiments. A.U.: arbitrary units.</p
<p>U937 cells were treated with LPS or ASTA at the doses indicated. Groups include the following: Nu...
<p>Cell lysates of PC14PE6/AS2-Vec(1), S3C(1), and S3C(2) (A) and cell lysates of Vec(1), S3D(1), an...
BEAS-2Bs were exposed to EPFR (DCB230 or MCP230) at 50 μg/cm2 or 100 μg/cm2 for 24h, and TNF-α produ...
<p>(A) Splenocytes were harvested 6 h after stimulation by PA-MSHA and lysate by RIPA buffer. Expres...
<p>(A, B) Soluble protein extracts from HeLa cells transfected with pro-WT were sequentially immunop...