Surface expression of TNF-α on RAW264.7 cells measured by flow cytometry, and TNF-α ELISA from cell culture supernatants of the same experiments.

<p>Results shown in <b>A</b> and <b>B</b> were obtained in one experiment and results shown in <b>C</b> and <b>D</b> were obtained in an independent experiment. In some groups, no TNF-α was detected (nd). Each group contained 5 samples. Two repeat experiments are shown. Cells were treated with ethanol (either 43.4 mM or 86.8 mM), 100 ng/ml LPS or 20 µg/ml TAPI-0, or a combination of these treatments. ELISA was performed from cell culture supernatants of each group. The percentage of gated cells (positive for TNF-α surface expression by flow cytometry) for each group is shown in <b>A</b> & <b>C</b>. The ELISA results from cell culture supernatants of the cells used for flow cytometry are depicted in <b>B</b> and <b>D</b>. Bars designated by the same letter are not significantly different (p>0.05); bars with no shared letters are significantly different (p<0.05). Histograms for representative samples from key groups for the experiment shown in A are shown below panels <b>C</b> and <b>D</b>.