THP-1 cell surface accumulation of membrane-bound TNF-α after Ro 32-7315 and TQ (Panel A) or Celastrol (Panel B) treatment.

<p>Cells were stimulated for 4 hours (1 µg/ml of LPS) in the presence of Ro 32-7315 (3.5 µM), TQ (10 µM) and Celastrol (10 µM) or 0.1% (v/v) DMSO as a control. The intact cells were then stained by FITC-anti-m-TNF-α MAb. Membrane-bound TNF-α expression was evaluated in flow cytometry. Representative histograms are shown. The fluorescence intensity (AU) is plotted versus the number of cells. For all 3 compounds, membrane –bound TNF-α expression increased after THP-1 cells were treated (p<0.05).