MK591 pre-treatment of RAW264.7 cells reduced LPS and Lipid A induced TNF-α secretion.

<p>RAW264.7 cells were pre-treated for 30 minutes with MK591 or vehicle following by LPS or Lipid A stimulation for 0.5 hr (A) and 4 hr (B). Conditioned media were collected and TNF-α levels analyzed by ELISA. Quantitative data are presented as means ± SEM from 6–7 independent experiments. *p<0.01 and ^†p<0.001 compared to LPS-treated cells and ^‡p<0.01 and ^§p<0.001 compared to Lipid A-treated cells.