Increased responsiveness of SP-R210<sub>L</sub>(DN) cells to LPS.
- Linlin Yang (737661)
- Marykate Carrillo (737662)
- Yuchieh M. Wu (737663)
- Susan L. DiAngelo (737664)
- Patricia Silveyra (737665)
- Todd M. Umstead (737666)
- E. Scott Halstead (737667)
- Michael L. Davies (197464)
- Sanmei Hu (737668)
- Joanna Floros (737669)
- Francis X. McCormack (31499)
- Neil D. Christensen (347949)
- Zissis C. Chroneos (737670)
Publication date
May 2015
DOIAbstract
<p>Control and SPR210<sub>L</sub>(DN) cells were plated in 12-well tissue culture dishes at a density of 150,000 cells/well and cultured 24 hrs in RPMI/10% FBS. Cells were then treated with 100 ng/mL LPS for 2, 4, 8, 16, and 24 hrs. A) Intracellular staining of TNFα was performed in brefeldin A blocked cells 2 hrs after treatment with LPS. Dotted histograms show untreated cells. Black, red, and blue histograms show intracellular TNFα staining of control, SP-R210<sub>L</sub>(DN)1, and SP-R210<sub>L</sub>(DN)2 cells. B) The levels of secreted TNFα were measured by ELISA in media at indicated time points after treatment with LPS. (n = 6; ***p<0.005).</p
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