Stat3 activation increased TF expression on cell surface in PC14PE6/AS2 cells.

<p>Cell lysates of PC14PE6/AS2-Vec(1), S3C(1), and S3C(2) (A) and cell lysates of Vec(1), S3D(1), and S3D(2) (B) were harvested and subjected to Western blot analysis to detect the expression levels of Stat3 and TF protein using various antibodies as indicated. (C) Vec (1), Vec(2), S3D(1), and S3D(2) cells were seeded on chamber slides for 24 h. To visualize cell surface TF expression, the cells were fixed without permeabilization and then stained with anti-TF antibody. Images were collected by confocal microscopy. (D) Vec(1), Vec(2), S3D(1), and S3D(2) cells were seeded on a 100 mm dish for 24 h. Cells were then fixed and stained with anti-TF antibody. Signals were analyzed by flow cytometry.