Effects of ASI, ASII and ASIII on TNF-induced ICAM-1 expression and IκBα degradation.

<p>Confluent mAECs were pre-incubated with 250 µg/mL ASI, ASII or ASIII for 2 h, then washed and incubated for 6 h or 30 min with 30 ng/mL TNFα. Cells were then collected and analyzed for the expression of ICAM-1 mRNA or IκBα by performing real-time qPCR and Western blot analysis, respectively. Data were representative of three independent experiments with determinations made in triplicate and shown as mean values+S.D. * P<0.05, **P<0.01 compared with cells treated with TNFα. CN: Control.