Effects of AST and ASIV on TNF-induced apoptosis.

<p>Confluent mAECs were pre-incubated with 250 µg/mL AST or ASIV for 2 h, then washed and incubated for 8 h with 30 ng/mL TNFα+10 µg/mL CHX (T/C). The cells were then photographed, collected and analyzed for the expression of cleaved-caspase 3 by Western blot analysis. Data were representative of three independent experiments with determinations made in triplicate and shown as mean values+S.D. * P<0.05, **P<0.01 compared with cells treated with TNF+CHX. CN: control; T/C: TNF+CHX.