miRNAs were differentially expressed in exosomes during osteogenic differentiation of human BMSCs.

<p>Heatmap representation of the mean fold change in osteogenic differentiation related differential miRNA signature. Two-dimensional grid matrix displaying 5 differential miRNAs (miR-199b, miR-218, miR-148a, miR-135b, and miR-221) was obtained by the functional heat-map in R. Columns refer to time course comparison: human BMSC culture at 0, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 7 days. Rows stand for the 5 differential miRNAs. Each entry of the grid refers to relative fold (log2) between the expression level of a given miRNA in exosome relative to RNU44 in human BMSCs. The color of each entry is determined by the value of that fold difference, ranging from red (negative values) to yellow (positive values).