Heat map of differentially expressed miRNAs, and analysis of functions and pathways corresponding to possible target genes.

(A) Heat map analysis of differentially expressed miRNAs in three groups (normal group N: Normal, acute inflammation model group A: Acute, chronic inflammation model group C: Chronic). Columns and rows represent samples and specific miRNAs. The miRNA cluster tree is shown on the left. The code on the legend is the value after log2 conversion. The color scale indicates the relative expression level of the miRNA. Red indicates that the expression of miRNA in the treated group is higher than that in the control group, while green indicates that the expression of the miRNA in the treated group is lower than that in the control group. Black indicates that there is no difference in miRNA between the two groups. (B) GO analysis of target genes predicted by differentially expressed miRNAs in the normal group and the acute inflammation model group. (C) GO analysis of target genes predicted by differentially expressed miRNAs in the normal group and the chronic inflammation model group. The GO category is shown on the x-axis and the number of genes is shown on the y-axis. Biological processes (BP) are a major class of GO analysis, including various processes associated with inflammation. Molecular function (MF) is rich in protein binding, zinc ion binding, transcription factor activity, and so on. The cellular component (CC) is enriched in the nucleus and is indispensable for membrane, cytoplasm and so on. (D) Analysis of target gene pathways predicted by differentially expressed miRNAs in the normal group and the acute inflammation model group. (E) Analysis of target gene pathways predicted by differentially expressed miRNAs in the normal group and the acute inflammation model group. The 15 most abundant signal transduction pathways were selected based on the P value (P < 0.01). The x-axis shows the signal path term and the y-axis shows the degree of enrichment.