Heat map showing the expression levels of the differentially expressed miRNAs.

<p>The differential expression analysis between the seed (S), the placenta (P), and the flesh (F) samples at (A) 28 DAA and (B) 40 DAA was conducted using DESeq2. The results were filtered to keep only those miRNAs whose mean normalized expression level was at least 10, and the expression change was at least six-fold. We colored the conservative (black), known (blue) and novel (red) miRNAs. We found 21 differentially expressed miRNAs between different stages (28, 40) in the flesh (C), 28 miRNAs in the seed (D), and none in the placenta. Arrows represent up- (↑) and down-regulation (↓) compared to the two other tissues (for example S↑ means, the expression of the marked miRNA are upregulated in seed compared to both, placenta and flesh. We also listed those changes, which occurred only between two tissues. For example, we marked a miRNA up-regulation between the placenta and the seed like this: P→S↑.